Stability and degradation of PRINT nanoparticles

COLL 200

Ji Guo, guoj@email.unc.edu1, Stephanie E. A. Gratton, gratton@email.unc.edu1, Patrick D. Pohlhaus, pdp@unc.edu1, Andrew Murphy2, Ashley Galloway2, Derek Schorzman2, and Joseph M. DeSimone, desimone@unc.edu3. (1) Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-3290, (2) Liquidia Technologies, Inc, (3) NSF Center for Environmentally Responsible Solvents and Processes, Department of Chemistry, University of North Carolina & Department of Chemical Engineering, NC State University, CB# 3290, 300 Venable & Kenan Laboratories, Chapel Hill, NC 27599-3290
A new particle generation technique, called PRINTTM (Particle Replication In Non-wetting Templates), enables the simultaneous and independent control over particle size and shape, composition, and surface functionality. The uniform structure and good stability of PRINT nanoparticles in neutral physiological environments (Phosphate Buffer, pH 7.0) was confirmed by dynamic light scattering (DLS). Preliminary degradation studies of different PRINT Trojan Horse nanoparticles were conducted in order to mimic the intracellular environment found in early to late endosomes which occur during the endocytosis of PRINT particles in a wide range of cell types. It was found that PRINT nanoparticles comprised of poly(lactic-co-glycolic acid) (PLGA) matrices degraded instantly under low pH environment. PRINT nanoparticles comprised of disulfide linkage also displayed biodegradability with existence of dithiothreitol.

 

Fundamental Research in Colloid and Surface Chemistry
6:00 PM-8:00 PM, Monday, August 20, 2007 BCEC -- East Registration, Poster

Division of Colloid & Surface Chemistry

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007