ANYL 95 |
| Multidimensional protein identification technology (MudPIT) is a powerful method for the identification of proteins. By combining two-dimensional chromatography with tandem mass spectrometry, proteins present in complex biological samples can be identified. MudPIT provides several advantages such as, fewer sample-handling steps, increased protein recovery, and better resolution of proteins that were previously difficult to recover like hydrophobic proteins and proteins with extreme isoelectric points or sizes. Our efforts focus on the determination of proteins in mouse urine that might elicit an olfactory response. We have analyzed multiple samples from male and female mice in various reproductive states, examining how initial sample concentration, sample preparation, and chromatographic gradients affect protein detection. Normalized spectral counting (NSAF) and frequency of detection among replicates was used to quantify proteins that were differentially expressed and may be olfactory markers. This presentation will focus on the determination of our analytical conditions and the resulting protein identifications. |
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General Posters
7:00 PM-9:00 PM, Sunday, August 19, 2007 BCEC -- Exhibit Hall - B2, Poster
Division of Analytical Chemistry |