COLL 3 |
| Introduction. Staphylococcus aureus enterotoxin B (SEB) is a highly heat resistant enteric toxin that is a potential a biothreat agent that can easily be dispersed through food and water distribution. A sensitive and reliable method for the detection of this toxin in water and in complex food matrix is needed. Method. Cantilever sensors were prepared by immobilizing polyclonal antibody specific to SEB and then exposed to food samples spiked with SEB of known concentrations in a flow cell arrangement. Results. Detection of 100 fg, 1 pg, and 10 pg SEB in apple juice resulted in resonance frequent y decreases of 113 „b 18, 308 „b 24, and 521 „b 20 Hz, respectively. Detection of the same quantities in milk resulted in resonance frequency decreases of 143 „b 35, 310 „b 32, and 557 „b 25 Hz, respectively. Positive detection of SEB in the sample solution was observed within the first 20-minutes. Positive, negative, and buffer control responses were -17 „b 10, -9 „b 5, and -6 „b 12 Hz, respectively. Positive verification of SEB attachment was confirmed by low-pH buffer release and second antibody binding post-detection. Conclusion. The cantilver sensors can reliably detect SEB at less than 100 fg in complex food matrices in 20 minutes, and could serve well in fight against bioterrorism. |
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Nanoscience and Nanotechnology for Chemical and Biological Defense
9:00 AM-12:00 PM, Sunday, August 19, 2007 BCEC -- 151B, Oral
Division of Colloid & Surface Chemistry |