ANYL 319 |
| Oxidative stress has long been hypothesized as an underlying mechanism associated in the etiology of many chronic diseases. Conventional methods for disease screening typically focus on single biomarker analyses associated with oxidative stress. In this study, in-vitro oxidation experiments of plasma were performed in order to assess global changes in metabolism by CE-ESI-MS. On-line preconcentration with desalting by CE-ESI-MS permitted direct analysis of deproteinized plasma samples without extensive sample pretreatment. This strategy was used to identify susceptible metabolites sensitive to oxidative stress, as well as assess overall antioxidant capacity reflecting the total protein and non-protein composition of plasma samples. The development of rapid screening methods for assessment of oxidative stress and antioxidant capacity represents a promising approach for early detection and intervention of chronic diseases, such as cardiovascular and neurodegenerative disorders. |
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Biomarker Discovery
1:30 PM-4:20 PM, Monday, August 20, 2007 BCEC -- 104A, Oral
Division of Analytical Chemistry |