BIOL 199 |
| Plantaginaceae has been proven to have a variety of bioactivity and wildly used for the antiinflammatory, immunomdulatory, and adjuvant of antitumor therapy in traditional medicine of the world. Acteoside and isoacteoside were isolated from seeds of Plantago asiatica L. The effect of both compounds on proliferation of murine bone marrow-derived dendritic cells in vitro was studied by using the tetrazolium salt reduction (MTT) assay. The results showed that both acteoside and isoacteoside could significantly stimulate the proliferation of dendritic cells, compared with the negative control (p<0.05). Acteoside in different concentrations (0.1µg/mL, 1µg/mL, 10µg/mL and 100µg/mL) all had better promoting effect than the positive control (rmGM-CSF+ rmIL-4) (p<0.05). It was also found that acteoside and isoacteoside at the concentrations of 0.1µg/mL to 100µg/mL coordinated with cytokine (rmGM-CSF+ rmIL-4) could significantly promote the effect of cytokine (p<0.05). However, both compounds at a very high concentration of 1000µg/mL had an inhibition effect on the proliferation of dendritic cells. These results will facilitate our further research to investigate the effect of acteoside or isoacteoside on the phenotypic and functional maturation of murine dendritic cells. Keywords: Plantago asiatica L; Acteoside; Isoacteoside; Dendritic cells; Proliferation. * Supported financially by National Natural Science Foundation of China (No: 30660226) and Program for Changjiang Scholars and Innovative Research Team in University (No: IRT0540) Corresponding author: Mingyong Xie, Ph.D in Nutrition, Professor in Food Science. |
|
Frontiers in Chemical Biology
5:00 PM-7:00 PM, Wednesday, August 22, 2007 BCEC -- Exhibit Hall - B2, Poster
Division of Biological Chemistry |