A HPLC method to determine potassium acetate concentration

ANYL 59

Tina Yu, yyu@aspus.jnj.com, R&D Chemistry, Advanced Sterilization Products, Johnson & Johnson, 33 Technology Drive, Irvine, CA 92618 and Xiaolan Chen, xchen1@aspus.jnj.com, Operation Chemistry, Advanced Sterilization Products, Johnson & Johnson, 33 Technology Drive, Irvine, CA 92618.
Potassium acetate is very polar and does not retain well on the conventional reverse-phase C-18 columns without adding ion-pairing reagent into the mobile phase. Waters Atlantis™ dC18 column is specifically designed to retain polar compounds, and therefore is used for the method development. During the analysis, acetate is converted to acetic acid under the mobile phase pH of 2.5. Detection wavelength is set at 215nm.

The method is implemented as a gradient method since the excipients require longer time to elute. The gradient programming provides sufficient retention for acetic acid, while shortens the total run time to 12 minutes. This method can also be applied isocratically with a three-minute run time to other applications where the excipients retention is not an issue.

The method is validated and demonstrated to be linear, precise, accurate, specific, and robust.

 

General Posters
7:00 PM-9:00 PM, Sunday, August 19, 2007 BCEC -- Exhibit Hall - B2, Poster

Division of Analytical Chemistry

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007