Use of ELISA to aid management of the invasive gall wasp, Quadrasticus erythrinae Kim on native and landscape trees, Erythrina spp

AGRO 4

Qing X Li, qingl@hawaii.edu1, Ting Xu, tingxucau@yahoo.com1, Christopher Jacobsen, postharvest@ctahr.hawaii.edu2, and Arnold H. Hara, arnold@hawaii.edu2. (1) Department of Molecular Biosciences and Bioengineering, University of Hawaii, 1955 East-West Rd., Honolulu, HI 96822, (2) Beaumont Agricultural Research Center, University of Hawaii, 875 Komohana Street, Hilo, HI 96720
The gall wasp Quadrastichus erythrinae Kim was first described as a new species infesting Erythrina variegata, E. fusca and E. indica in 2004 in Singapore, Mauritius and Reunion, and soon after reported infestations on five Erythrina species in Taiwan. In April 2005, the wasp was first detected on the island of Oahu and rapidly established on five islands in Hawaii by August 2005. In Hawaii, about all Erythrina trees including the endemic wiliwili tree, E. sandwicensis are now infested. After a number of insecticides applied by drench, spray and injection were tested, imidacloprid by injection was selected for further studies due to promising control efficacies of the wasp. An ELISA was evaluated for its reproducibility, accuracy, matrix interference, recoveries, and comparability to HPLC results for the analysis of imidacloprid in wiliwili trees. An inverse relationship between the leaf infestation severity rating and imidacloprid concentrations shows that imidacloprid can control the wasps.