INOR 143 |
| Inorganic complexes are commonly used for sensing and imaging in biological systems. An ongoing challenge is the optimization of these complexes to provide higher selectivity, specificity, and sensitivity because many bind to nucleic acids and proteins non-specifically. Our approach is to develop multimetallic architectures that are tethered to a polyamide strand and have, because of their polyvalency, increased binding affinity to oligonucleotides. The artificial oligopeptides are prepared using solid phase peptide synthesis methods and these are reacted with Cu (II) and Pt (II) complexes to form the multimetallic structures. These are fully characterized by NMR and mass spectrometry, as well as absorption and emission spectroscopy and electrochemical methods. Binding of the multimetallic strand with double-stranded DNA is monitored by fluorescence titrations and circular dichroism, and the stoichiometry and thermodynamic parameters quantitatively determined by ITC. Our results suggest that our tethered multimetallic systems may find use as new luminescent sensors to monitor DNA binding events that may be useful for in vitro diagnostics. |
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Bioinorganic Chemistry: DNA and RNA
1:30 PM-5:30 PM, Sunday, August 19, 2007 BCEC -- 211, Oral
Division of Inorganic Chemistry |