Calibration of microdialysis probes during long term implantation

ANYL 78

Xiaodun Mou, moux@rpi.edu, Chemistry and Chemical Biology, Rensselaer Polytechnic Institute, Cogswell Lab, Rensselaer Polytechnic Institute, 110 8th Street, Troy, NY 12180, Michelle R. Lennartz, lennarm@mail.amc.edu, Center for Cell Biology & Cancer Research, Albany Medical College, 47 New Scotland Avenue, Albany, NY 12208, Daniel Loegering, Center for Cardiovascular Sciences, Albany Medical College, 47 New Scotland Ave, Albany, NY 12208, and Julie A. Stenken, stenkj@rpi.edu, Department of Chemistry and Chemical Biology, Rensselaer Polytechnic Institute, 228 Cogswell, 110 8th Street, Troy, NY 12180.
The analytical performance of in vivo sensor implants is a crucial parameter to understand. Microdialysis sampling probes implanted in vivo into male Sprague-Dawley rats were used to determine how the foreign body response affects probe calibration. Dialysis probes with either a polycarbonate (PC) or polyethersulfone (PES) membrane were implanted. Vitamin B12 and antipyrine were internal standards to test the membrane and capillary permeability towards different sized molecules, and 2-deoxyglucose was employed to trace the glucose metabolism variation during the elicited foreign body response. Histological staining was applied to surrounding tissue seven (PC) and twelve days (PES) after implantation. The capsule surrounding the probe blocked the diffusion of relatively large molecule (Vitamin B12) and caused the decrease in glucose collection by cutting of the blood supply. However, it did not affect the local delivery of antipyrine or 2-deoxyglucose (Table 1). NIH EB001441 is acknowledged for funding.

 

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7:00 PM-9:00 PM, Sunday, August 19, 2007 BCEC -- Exhibit Hall - B2, Poster

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Division of Analytical Chemistry

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007