Synthesis and application of nucleoside analogs to study RNA-editing and RNA interference

BIOL 252

Olena Maydanovych, maydanov@chem.utah.edu, Department of Chemistry, University of Utah, 315 South 1400 East, Salt Lake City, UT 84112-0850 and Peter A Beal, beal@chem.ucdavis.edu, Department of Chemistry, University of California, Davis, One Shields Avenue, Davis, CA 95616.
The synthesis of modified nucleoside analogs is one of the approaches our laboratory uses to aid in the understanding of biological processes. We present the synthesis of a wide range of nucleoside analogs, along with their application in the study of RNA-editing adenosine deaminase and RNA interference. Specifically, we are interested in finding a suitable active site binding ligand based on 8-aza-7-deazapurine ribonucleoside and preparing modified RNA substrates to elucidate the mechanism of RNA-editing catalyzed by adenosine deaminase. Additionally, we are focusing our attention on the synthesis of N2-alkyl derivatives of 2-aminopurine ribonucleoside by using substitution chemistry with 2-bromopurine precursor. The valuable combination of phosphoramidite synthesis and generation of necessary RNA oligonucleotides will help us to evaluate the effect of introduced modifications on RNA interference.
 

Frontiers in Chemical Biology
5:00 PM-7:00 PM, Wednesday, August 22, 2007 BCEC -- Exhibit Hall - B2, Poster

Division of Biological Chemistry

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007