ORGN 151 |
| Modified oligodeoxynucleotides (ODNs) have been used as potential therapeutic and diagnostic agents and in the study of several biochemical processes in nucleic acid research. Recently we reported the synthesis of modified ODNs containing self-complementary sequences or sequences with one kind of nucleosides. In the modified ODNs, the nucleosides were linked through 3',5'-diphosphodiester bridges. Herein, we report the synthesis and the duplex formation ability of non self-complimentary ODNs containing mixed sequences of four nucleoside bases that are attached through diphosphodiester internucleotide linkages. The modified ODNs were 12-mer complementary mixed sequences including, 5'-d(TTCTAATTGTAT), 5'-d(ATACAATTAGAA), 5'-d(ATGTCAACTGGT), 5'-d(ACCAGTTGACAT), 5'-d(CCATTGGAAACT), 5'-d(AGTTTCCAATGG), 5'-d(AACCTGATTGCA), and 5'-d(TGCAATCAGGTT). Modified-modified, modified-unmodified, and unmodified-unmodified mixtures of ODNs were studied and compared using CD spectropolarimetry, UV thermal denaturation (Tm) analysis, thermodynamic calculations, and nuclease stability studies in the presence of endonulease DNase I and 3'-exonuclease I. These studies confirmed that the modified ODNs with diphosphodiester bridges were able to bind to their complementary modified or unmodified ODNs. Modified-modified ODNs were stable toward nuclease enzymes.
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Total Synthesis, Materials, Devices and Switches, Molecular Recognition and Self-Assembly, Biologically-Related Molecules and Processes
8:00 PM-10:00 PM, Sunday, August 19, 2007 BCEC -- Exhibit Hall - B2, Poster
Division of Organic Chemistry |
