CARB 77 |
| We reported that a well-characterized glycoprotein fraction containing fucose residues in an extract of Ganoderma lucidum polysaccharides (Reishi, EORP) exerts certain immuno-modulation activity by stimulating the expression of inflammatory cytokines via TLR4. Here, we demonstrated that EORP increases the surface expression of CD14 and TLR4 within macrophages J774A.1 cells in vitro. Moreover, we observed the co-localization of internalized LPS with lysosome- and Golgi-apparatus markers within 5min after J774A.1 cells stimulated with LPS. In addition, EORP pretreatment of J774A.1 cells and human blood-derived primary macrophages, followed by LPS stimulation, results in the super-induction of interleukin-1beta (IL-1) expression. Our current results could provide a potential EORP-associated protection mechanism for bacteria infection by enhancing IL-1 expression and the clearance of contaminated LPS by macrophages. |
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General Posters
6:00 PM-8:00 PM, Tuesday, August 21, 2007 BCEC -- Exhibit Hall - B2, Poster
Sci-Mix
Division of Carbohydrate Chemistry |