Determination of optimum conditions for removal of bisphenol A by horseradish peroxidase and their application for removal of other derivatives

ENVR 129

Kazunori Yamada, k5yamada@cit.nihon-u.ac.jp, Naoya Ikeda, c65030@cit.nihon-u.ac.jp, Yoko Takano, Ayumi Kashiwada, Kiyomi Matsuda, and Mitsuo Hirata. Department of Applied Molecular Chemistry, College of Industrial Technology, Nihon University, 1-2-1 Izumi-cho, Narashino, Chiba, 275-8575, Japan
The optimum conditions such as hydrogen peroxide concentration, pH value, temperature and poly(ethylene glycol) (PEG) concentration were determined for removal of bisphenol A (BPA) by horseradish peroxidase (HRP) and this procedure was applied for removal of different bisphenol derivatives. BPA was completely removed with HRP at 1.0 U/ml in the presence of hydrogen peroxide and 10K-PEG at pH 6.0 and 30 ºC through the formation of oligomer precipitates. Eleven kinds of bisphenol derivatives were effectively removed by increasing the HRP dose as necessary under the optimum conditions determined for removal of BPA. Since the aggregation of oligomer precipitates was effectively enhanced by adjusting the pH value with HCl to 4.0 after the enzymatic treatment, transparent and colorless solutions were obtained by filtration and % removal values of 96-100% were obtained. This paper demonstrated that BPA and its derivatives were highly removed with HRP.
 

General Papers
6:00 PM-8:00 PM, Wednesday, August 22, 2007 BCEC -- Exhibit Hall - B2, Poster

Division of Environmental Chemistry

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007