BIOT 1 |
| The nondestructive nature of cellular behavior analysis using long-term imaging facilitates cell classification. To look for new indicators of self-renewing capacity, hematopoietic stem cells (HSCs) isolated from adult mouse marrow were individually micromanipulated into the wells of a microarray imaging chamber and then cultured for 4 days with each cell and its progeny being imaged using time-lapse photography. Individual clones were then harvested and assayed in mice for HSC compartment repopulating activity. Characteristics identified from image sequences were screened and 3 were found to be predictive of HSC potential: mitotic activity, longer cell cycle times, and uropodia. Clone selection criteria developed using these data allowed all HSC-containing clones to be detected and at a 2.4-fold greater efficiency in subsequent experiments. The BACS platform represents an advance towards the goal of dynamic single cell analysis in heterogeneous populations and demonstrates the potential of this technology for preparative cell isolation devices. |
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Stem Cells: Quantifying, Modeling and Controlling Stem Cell Fate
8:20 AM-10:40 AM, Sunday, August 19, 2007 BCEC -- 108, Oral
Division of Biochemical Technology |