BIOL 207 |
| β-mannanase, a versatile enzyme, can be prepared by fermentation and has been widely used in the food, feed, oil and paper industries. In order to analyze and optimize metabolic pathway, a simplified metabolic network which contains 38 main biological reactions and 37 key intermediates and products, was constructed for production of β-mannanase from Bacillus licheniformis. A balance-based metabolic flux model was proposed with the assumption of pseudo steady state for the intermediates. By using linear programming method, the optimal flux distributions within the network were determined via maximizing or minimizing some objective functions, e.g. the rate of enzyme synthesis, bacterial growth as well as NADH production. When β-mannanase production was selected as the objective function, the maximum yield of β-mannanase was theoretically calculated as 59.82% in terms of mannose mol amount. According to the FBA method, it was proved that optimizing bacterial growth was equivalent to minimizing NADH production. Therefore, it could be demonstrated that the effect of TCA on enzyme production was more significant than that on bacterial growth based on the calculated results i.e. the metabolic flux of TCA was increased from 9.45% of maximizing bacterial growth to 51.25% of maximinzing β-mannanase production.
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Frontiers in Chemical Biology
5:00 PM-7:00 PM, Wednesday, August 22, 2007 BCEC -- Exhibit Hall - B2, Poster
Division of Biological Chemistry |
