Biomineralization studies of carboxymethyl chitosan-graft-D-glucuronic acid membranes for tissue engineering applications

CARB 72

Rangasamy Jayakumar, jayakumar77@yahoo.com, Faculty of Chemistry, Materials and Bioengineering, and HRC, Kansai University, Japan, Suita, Osaka, 564-8680, Japan and Hiroshi Tamura, tamura@ipcku.kansai-u.ac.jp, Faculty of Chemistry, Materials and Bioengineering, and HRC, Kansai University, Suita, Osaka, 564-8680, Japan.
Graft copolymerization onto carboxymethyl chitosan (CM-Chitosan) will be a key point, which will introduce desired properties and enlarge the potential applications of chitosan in tissue engineering. For this purpose, CM-chitosan-graft-D-glucuronic acid (GA) membranes were prepared from the grated CM-chitosan-graft-GA and carried out the preliminary biomineralization studies by using biomimetic method. The prepared membranes were immersed in 1.5 x SBF solutions for 7, 14 & 21 days after CaCl2 treatment for 3 days at 37 ºC in order to enhance the bioactivity. After 7, 14 & 21 days, the CM-chitosan-graft-GA membranes induce the formation of a bone-like apatite layer on their surface. The formations of apatite layer were also confirmed by scanning electron microsscopy(SEM), confocal laser scanning microscopy(CLSM), thin film X-ray diffraction (TF-XRD) & FTIR analysis. These results revealed that CM-chitosan-graft-GA has showing bioactivity and it can be potentially applicable in bone tissue repair and regeneration.

 

General Posters
6:00 PM-8:00 PM, Tuesday, August 21, 2007 BCEC -- Exhibit Hall - B2, Poster

Division of Carbohydrate Chemistry

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007