Determination of phenolic components and deduction of the fragmentation mechanism of danshen by HPLC/electrospray ionization-multistage mass spectrometry

ANYL 102

Wei Qi, qiwei@tju.edu.cn, Honglei Wang, and Zhimin He, enzyme@tju.edu.cn. School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China
Danshen (Salvia mitiorrhiza bunge) is an important and popular traditional Chinese medicine for improving microcirculation, dilating coronary arteries and preventing myocardial ischemia. The Compound Danshen Dripping Pill (CDDP) has been authorized to be tested clinically in the United States by FDA in 1997. In this work, the multi-component fingerprint of Danshen was studied and the main bioactive components of CDDP, such as Danshensu and Salvianolic B, were identified via reverse HPLC /Multistage Mass Spectrometry. The electrospray ionization (ESI) -MS-MS spectra of most salvianolic acids displayed a characteristic behavior of neutral losing one molecule of danshensu. The mechanism for fragmentation of the negative ion of peak 1 (m/z 717) was studied in details by MS3. Moreover, the MS3 data of peak 1 indicates that it contains Salvianolic B and Salvianolic E, which would not be identified either by comparing the retention time or analyzing by MS-MS.

 

General Posters
7:00 PM-9:00 PM, Sunday, August 19, 2007 BCEC -- Exhibit Hall - B2, Poster

Division of Analytical Chemistry

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007