Comparison of automated potentiometric titration and high-performance liquid chromatography for the determination of peracetic acid and hydrogen peroxide

ANYL 87

Qiang Li, QLi1@aspus.jnj.com and Tina Yu, YYu@aspus.jnj.com. Biocides Research & Development, Advanced Sterilization Products, a Johnson and Johnson Company, 33 Technology Drive, Irvine, CA 92618
In this study, an automated potentiometric titration and a high-performance liquid chromatography (HPLC) were used to analyze the concentrations of peracetic acid (PAA) and hydrogen peroxide (H2O2). For a two-step titration, the concentration of H2O2 was first determined by cerium (IV) through redox reaction. PAA was then titrated with iodine / thiosulfate. For HPLC method, the quantitative reaction of PAA with methyl p-tolyl sulfide and H2O2 with triphenylphosphine results in the corresponding sulfoxide and phosphine oxide, which can be separated on a reversed-phase column and detected by ultraviolet detector at 230 nm. Both automated titration and HPLC were proved to be viable for analysis of PAA and H2O2. The HPLC method is characterized by a high reproducibility and a low detection limit but it is time-consuming. Automated titration, the direct determination method, is ideal for the analysis of PAA and H2O2 at higher concentrations without interference of background.
 

General Posters
7:00 PM-9:00 PM, Sunday, August 19, 2007 BCEC -- Exhibit Hall - B2, Poster

Division of Analytical Chemistry

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007