BIOT 137 |
| Oxidation represents a major degradation pathway for protein formulations in solutions and solids. The parameters controlling protein oxidation are not as well understood as it would be desirable for the rational design of oxidation-resistant formulations. An important feature of the reaction of a protein with a reactive oxygen species/free radical is that the initial site of attack may not necessarily represent the final location of an oxidation site. In this presentation, we will demonstrate the potential of Cys residues to serve as a point of primary attack with intermediary Cys thiyl radicals subsequently attacking other amino acids within the protein sequence. Thiyl radicals can either involve in reversible hydrogen transfer reactions, electron transfer, or in addition reactions to aromatic amino acid residues. Experimental evidence will be provided for each of these pathways. |
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Biophysical and Biomolecular Symposium: Protein Chemical Instability
8:00 AM-11:05 AM, Tuesday, August 21, 2007 BCEC -- 106, Oral
Division of Biochemical Technology |