On-chip investigations of specific ion effects on polymer and protein aggregation

ANYL 378

Yanjie Zhang, yzhang@mail.chem.tamu.edu, David E. Bergbreiter, bergbreiter@tamu.edu, and Paul S. Cremer, colloid.program.chair@mail.chem.tamu.edu. Department of Chemistry, Texas A&M University, MS 3255, College Station, TX 77843
A wide variety of physical processes from protein folding and enzymatic activity to colloidal assembly and crystallization follows a recurring trend now known as the Hofmeister series. In this presentation, temperature gradient microfluidics is employed to investigate specific ion effects on the phase transition of thermoresponsive polymers and peptides and the clouding point of proteins. Our assays enable high-throughput measurements to be made on the physical behavior of these species with high temperature precision. The mechanism of the Hofmeister effect will be discussed.
 

Analytical Approaches
9:00 AM-12:00 PM, Wednesday, August 22, 2007 BCEC -- 104B, Oral

Division of Analytical Chemistry

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007