AEI 85 |
| Chemical probes that target specific classes of proteins based on shared functional properties have emerged as powerful tools for proteomics. The metabolome rivals, if not surpasses, the proteome in terms of size and complexity, suggesting that efforts to profile metabolites would also benefit from targeted technologies. We applied the principle of chemoselective probes to the metabolome, creating a general strategy to tag, enrich, and profile large classes of small molecules from biological systems. Key to success was the incorporation of a protease cleavage step to release captured metabolites in a format compatible with direct liquid chromatography-mass spectrometry analysis. This technology, referred to as Metabolite Enrichment by Tagging and Proteolytic Release (METPR), is applicable to small molecules of any physicochemical class (e.g., polar, labile, low mass). We applied METPR to profile cancer cells treated with the antioxidant N-acetyl-L-cysteine, where the method accurately reported induced changes in the thiol metabolome. |
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Academic Employment Initiative
8:00 PM-10:00 PM, Monday, August 20, 2007 BCEC -- Exhibit Hall - B2, Sci-Mix
Academic Employment Initiative |