Spectroscopic response of complexometric indicators with bimetallic enzyme model complexes and their application as dye displacement anion sensor

INOR 215

Brad P. Morgan, bradm@clemson.edu1, Susan He1, and Rhett C. Smith, rhett@clemson.edu2. (1) Department of Chemistry, Clemson University, 479 Hunter Labs, Clemson, SC 29634, (2) Department of Chemistry, Center for Optical Materials Science and Engineering Technologies (COMSET), Clemson University, 479 Hunter Labs, Clemson, SC 29634
Although the optical response of complexometric dyes to single metal ions in solution has been well-studied, characterization of similar interactions with bimetallic centers remains underexplored. The current work focuses on elucidating the interaction of well-known and commercially-available complexometric dyes with dizinc phosphohydrolase model compounds utilizing 2,6-Bis-[(di(2-picolyl)amino)-methyl]-4-methyl-phenol and related ligands. The binding affinity of dizinc complexes have been spectroscopically determined (UV-vis and/or fluorescence) for eighteen commercially available complexometric dyes, and the dependence of binding affinity on pH, buffer composition, and solvent have been explored. Binding data were subsequently used to guide the development of dye-displacement sensors for phosphate and pyrophosphate recognition as a first step towards the rational design of dye-displacement assays for biologically important anions.
 

General Inorganic Chemistry
7:00 PM-10:00 PM, Sunday, August 19, 2007 BCEC -- Exhibit Hall - B2, Poster

Division of Inorganic Chemistry

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007