COLL 28 |
| A method based on the use of attenuated total reflection-Fourier transform infrared (ATR-FTIR) has been developed to study the membrane fluidity in liposomes. Liposomes are fabricated with entrapped probe molecules and then adsorbed onto a TiO2 coated ATR crystal. The decrease in the IR bands of the probe molecule is due to leakage of the probe molecule from the liposomes and this is correlated to the packing density and degree of hydration determined by frequency position and bandwidth of IR bands due to the alkyl chain and headgroup of the phospholipid. The effect of cholesterol and UV crossliking of the alkyl chain on membrane fluidity were studied. It is shown that both cholesterol and UV polymerization reduce the permeability of eggPC liposomes. In addition, that the IR spectra show that cholesterol increases the headgoup hydration and decrease interchain order within the liposome bilayer, whereas UV polymerization contributes to a higher hydration level in both headgroup and interchain region.
|
|
Surfactant Self-Assembly
9:00 AM-12:15 PM, Sunday, August 19, 2007 BCEC -- 153B, Oral
Division of Colloid & Surface Chemistry |
