BIOT 70 |
| PEI/DNA nanoparticles has recently been used to make nonviral vectors for gene delivery. The conventional method of producing this nanoparticles involve mixing the ingredients together in bulk followed by vortexing which result in large particle size, low cytotoxicity, and poor gene transfection at low N/P ratios whereas small particle size, high cytotoxicity, and better gene transfection at high N/P ratios. Recently, the use of microfluidic channels with hydrodynamic focusing has generated well-defined and enhanced diffusional mass-transfer at the interfacial region between two fluids between two fluids for forming liposome particles. In this study, we used a microfluidic hydrodynamic focusing (MHF) device to achieve small nanoparticle size with narrow size distribution, and lower cytotoxicity for more efficient and consistent in vitro and in vivo gene transfection. The nanoparticles were characterized by atomic force microscopy (AFM), dynamic light scattering, and zeta potential. We have successfully demonstrated that nanoparticles produced by MHF yielded better gene expression in addition to smaller nanoparticle size and narrower size distribution at N/P=3.3.
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Emerging Technologies: Nanobiotechnology
8:00 AM-10:55 AM, Monday, August 20, 2007 BCEC -- 108, Oral
Division of Biochemical Technology |
