BIOT 251 |
| The emergence of transgenic crops as alternative production hosts for therapeutic proteins presents unique challenges for downstream process development. Each plant host contains a unique mixture of impurities (plant protein, lipids, phenolics, alkaloids, etc.) that must be removed during purification of the target protein. The presence and quantity of these impurities in the extract could vary significantly with extraction conditions such as the solid-to-liquid ratio, pH, buffer ionic strength, and homogenization method. In some situations, specific impurities (e.g. proteases and phenolics) dictate the sequence of unit operations and operating conditions. Key tasks in downstream process development are to identify critical extract impurities, evaluate their impact on selected recovery and purification steps, and to rationally design an integrated process that exploits potential advantages of each host system. This presentation will address strategies that could be employed in the recovery of pharmaceutical proteins from seed and leafy plant tissues. The potentially detrimental effects of plant proteases and the identification and removal of interfering phenolics from Lemna minor, rice, and tobacco extracts will also be discussed. |
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Poster Session
5:30 PM-7:30 PM, Wednesday, August 22, 2007 BCEC -- Exhibit Hall - B2, Poster
Division of Biochemical Technology |