BIOT 240 |
| The steroids dehydroepiandrosterone, pregnenolone, testosterone, progesterone, prednisone, cortisone, estrone and sarsasapogenin were fed to growing cultures of Exophiala jeanselmei var. lecanii corni. With 3Β-hydroxy-Δ5-steroids, dehydroepiandrosterone and pregnenolone there was hydroxylation at C-7. Alternately, these compounds were oxidized to the 3-ketone; the 5,6-double bond isomerised into conjugation; the olefin was then reduced and the 3-ketone was converted to the 3Α-alcohol. For the Δ4-3 keto steroids testosterone and progesterone both the double bond and carbonyl were reduced to the 3Α-hydroxy analogue and position 5 was hydroxylated. Cortisone and prednisone underwent reduction of the C-20 ketone only to give the 17Α,20S,21 triol, while estrone was simply hydroxylated at C-15. Sarsasapogenin remained untransformed. |
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Poster Session
5:30 PM-7:30 PM, Wednesday, August 22, 2007 BCEC -- Exhibit Hall - B2, Poster
Division of Biochemical Technology |