BIOT 98 |
| The role of a human stromal-based (hu-ST) serum-free culture system on the ex-vivo expansion/maintenance of hematopoietic stem/progenitor cells from adult bone marrow (BM) and umbilical cord blood (CB) was investigated. Significant expansion of BM and CB cells occurred in the hu-ST system. In stroma-free cultures, BM cells showed a significant lower expansion, whereas CB cells simply could not be expanded. A simple kinetic modeling study using two parameters (expansion rate and death rate), allowed to conclude that total cell expansion is similar for hematopoietic cells from both BM and CB, whereas cell death seemed to be related with the presence/absence of hu-ST ex vivo. A predictive model was also developed accounting for hematopoietic cell expansion, differentiation, and death, which estimated adequately total cell numbers and relative amounts of the different phenotypes (e.g. CD34+, CD34+CD38-, CD33+), allowing the prediction of expansion/differentiation pathways and identification of key steps in the hematopoiesis scheme. |
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Stem Cells: Engineering the Embryonic and Adult Stem Cell Niche
3:00 PM-5:00 PM, Monday, August 20, 2007 BCEC -- 108, Oral
Division of Biochemical Technology |