BIOT 255 |
| It has been well documented that different cell lines have unique nutritional requirements for maximum protein production. With current regulatory standards, undefined components, such as serum, have historically been eliminated from formulations. Additionally, process development timelines and production schedules do not always allow for a complete optimization of a chemically defined medium, feed strategy, and process. Therefore, the supplementation of a chemically defined medium with animal free hydrolysates has become a more prevalent and productive industry practice. Our unique media and process development procedures were utilized to achieve a significant increase in huIgG production. An initial productivity improvement was achieved through DOE-based chemically defined medium optimization. The production level was further increased by extending the work to include our DOE-based approach to peptone supplementation and process development. Feed media optimization and strategy development were further investigated in order to improve production in both shaker flask and bioreactor. |
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Poster Session
5:30 PM-7:30 PM, Wednesday, August 22, 2007 BCEC -- Exhibit Hall - B2, Poster
Division of Biochemical Technology |