Dual growth factor loaded PEGylated fibrin gels to control SMC phenotype from human MSC

BIOT 451

Charles T. Drinnan, c.drinnan@mail.utexas.edu and Laura J. Suggs. Department of Biomedical Engineering, University of Texas at Austin, 1 University Station, Mail Code C0800, Austin, TX 78712
The aim of the current study is to admix PDGF-BB and sequester TGF-β within a fibrin gel crosslinked with a bifunctional succinimidyl α-methylbutanote PEG (PEG-(SMB)2). This system can be utilized to modulate expression of smooth muscle phenotypes from a population of mesenchymal stem cells (MSC) by temporally controlling growth factor release. Gels are formed by thrombin addition to non-PEGylated gels, gels PEGylated with PEG-(SMB)2, and gels PEGylated with monofunctional mPEG-SMB. Controls consist of gels without growth factors. Release curves are quantified with commercial ELISA kits. TGF-β and PDGF-BB bioactivity is assessed with the Mv1Lu growth inhibition assay and a MSC migration assay, respectfully. Differentiation potential is measured using rtPCR with primers for smooth muscle α-actin and calponin. Results indicate that TGF-β is sequestered within the PEGylated gels while PDGF-BB is completely released by day 2. Further results demonstrate that released TGF-β maintains bioactivity.
 

Upstream Processing: Advances in Tissue Engineering
8:00 AM-11:00 AM, Thursday, August 23, 2007 BCEC -- 107C, Oral

Division of Biochemical Technology

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007