BIOT 14 |
| The molecular stability of proteins in association with a delivery system is a key consideration in the formulation of vaccines. The effect of adsorption and release of antigenic proteins from aluminum salts, PLG microparticles, MF59 suspensions can enhance the immunogenicity of the overall vaccine system due to adjuvant effects. We will examine the effects of formulating various proteins such as HIV-envelope, rPA for anthrax, hemagglutinin for influenza and Meningitis B on the overall stability and biophysical properties of the system. Spectroscopic and analytical methods including circular dichroism, fluorescence, light scattering, FTIR and chromatography were employed to measure changes in association, molecular structure, disposition and stability that occur to these important protein antigens in various delivery systems. |
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Biophysical and Biomolecular Symposium: Protein Stability
8:00 AM-11:10 AM, Sunday, August 19, 2007 BCEC -- 107 A/B, Oral
Division of Biochemical Technology |