BIOT 256 |
| To aid in the development of a more efficient CHO cell culture process and to monitor production, rapid feed back on the state of cell culture process is crucial. There is a need for on-line methods for the characterization of bioprocesses that will enhance process analysis and control. Monitoring of bioprocesses requires sensors providing real-time measurement of several variables to analyze, model, and control optimally the time course of these processes in bioreactors. Raman spectroscopy is a promising tool for the rapid, non-invasive, and multi-parameter analysis of aqueous biological systems. Additionally it may be considered as an essential element for PAT strategy. The potential of non-invasive and at-line determination of glucose, lactate, glutamine, ammonia, total cells, viable cells and protein titer in a CHO cell culture was investigated using Raman spectroscopy. Samples obtained from a fed batch bioreactor were analyzed with traditional methods and Raman spectroscopy. The Raman spectra were interpreted by using suitable spectra wavenumber regions through multivariate statistical techniques such as partial least square (PLS) and principal component regression (PCR). The correlation coefficient (R2) values for the PLS-1 calibration models for glucose, lactate, glutamine, ammonia, viable cells and total cells were greater than 0.9, where as, protein titer had a correlation coefficient of 0.84. Results indicated that Raman spectroscopy could be used for rapid detection of glucose, lactate, glutamine, ammonia, total cells, viable cells and protein titer in a CHO cell culture. |
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Poster Session
5:30 PM-7:30 PM, Wednesday, August 22, 2007 BCEC -- Exhibit Hall - B2, Poster
Division of Biochemical Technology |