Affinity adsorption of viruses using small peptide ligands

BIOT 78

Caryn L Heldt, clheldt@ncsu.edu1, Patrick V. Gurgel2, Lee-Ann Jaykus, leeann_jaykus@ncsu.edu3, and Ruben G Carbonell, ruben@ncsu.edu1. (1) Department of Chemical and Biomolecular Engineering, North Carolina State University, 911 Partner's Way, Raleigh, NC 27695, (2) ProMetic Life Sciences, 8168 chemin Montview, Mont-Royal, QC H4P 2L7, Canada, (3) Department of Food Science, North Carolina State University, Schaub Food Science Building 339A, Campus Box 7624, Raleigh, NC 27695
The capture of viruses from complex sample matrices is necessary for many different applications, including the removal of viruses from process streams, concentration of viral vectors for gene therapy, and molecular surface detection for use in a sensor. Small peptides can be used to create specific recognition surfaces and are a novel approach for the capture of viruses. For example, peptide sequences as small as three amino acids in length have been identified for the capture of porcine parvovirus (PPV). These peptides have been used in chromatographic columns for removal of PPV from complex mixtures containing human blood plasma. This approach is a promising alternative to virus capture using antibodies because it eliminates the risk of viral contamination that may be found in antibody preparations. Furthermore, peptides are inexpensive to produce and more stable than antibodies when used in a continual processing and regeneration environment.
 

Downstream Processing: Advances in Chromatography
8:00 AM-11:00 AM, Monday, August 20, 2007 BCEC -- 107 A/B, Oral

Division of Biochemical Technology

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007