Considerations for large scale extraction of monoclonal antibodies targeted to different subcellular compartments in transgenic tobacco plants

BIOT 102

Sally Hassan, sally.hassan@ucl.ac.uk1, Craig van Dolleweerd2, Julian Ma2, Wei Liu3, Colin R Thomas3, and Eli Keshavarz-Moore1. (1) Department of Biochemical Engineering, University College London, Torrington Place, London, WC1E 7JE, United Kingdom, (2) Department of Cellular and Molecular Medicine, St.George’s University of London, Cranmer Terrace, London, SW17 0RE, United Kingdom, (3) Department of Chemical Engineering, University of Birmingham, Edgbaston, Birmingham, B15 2TT, United Kingdom
Large scale purification of monoclonal antibodies from transgenic tobacco plants offers many advantages over commonly used host production systems. However, there is limited information on purification of pharmaceuticals from transgenic plants.

Methods for extraction of recombinant monoclonal antibodies from transgenic tobacco were investigated. Parameters for optimal extraction have been established, using techniques at small scale that will provide information for large scale purification. Three targeting strategies for antibodies in transgenic tobacco have been investigated in order to compare extraction from different subcellular compartments.

We have identified that antibody yield is dependent upon plant age, leaf position, pre-harvest wounding, extraction methodology, and extraction buffer composition, e.g. pH and detergent. These factors all influence early decisions on the best strategy for extraction of antibodies from plants. In addition, we have demonstrated that antibody yield from GM plant roots is similar to that from leaves, and is therefore a feasible alternative to leaves.

 

Downstream Processing: Bioprocess Integration & Industrial Case-Studies
3:00 PM-5:20 PM, Monday, August 20, 2007 BCEC -- 107 A/B, Oral

Division of Biochemical Technology

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007