BIOT 275 |
| The ribosome is a highly sophisticated RNA machine for the mRNA-templated amino acid oligo/polymerization to yield their size-, shape-, and sequence-defined peptides/proteins. Following the early demonstration of adaptor hypothesis, there has been much current challenge on applying the ribosomal decoding system for the synthesis of peptidomimetic libraries. However, the ribosome has strict limitations on substrates with elongated backbones. We show an unexpected loophole in the E.coli translation system. Nonsense suppression method revealed that the prokaryotic ribosomal system can accept main-chain elongated substrates if the pKa of the substrate was appropriately chosen. The factors governing the incorporation of main-chain elongated substrates will also be discussed. |
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Poster Session
5:30 PM-7:30 PM, Wednesday, August 22, 2007 BCEC -- Exhibit Hall - B2, Poster
Division of Biochemical Technology |