Characterization of direct immobilization on functionalized diamond SGFETs for detecting single-mismatch oligonucleotides

ANYL 443

Jung-Hoon Yang, junghoon_yang@moegi.waseda.jp, Consolidated Research Institute for Advanced Science and Medical Care, Waseda University, 513 Waseda Tsurumaki-cho, Shinjuku-ku, Tokyo, 162-0041, Japan, Shouma Kuga, Department of electrical engineering and bioscience, Waseda university, 3-4-1 Okubo, Shinju-Ku, Tokyo, 169-8555, Japan, and Hiroshi Kawarada, School of Science and Engineering, Waseda University, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo, 169-8555, Japan.
Diamond has a number of advantages for biomaterial detection, such as its wide potential window, simple functionalization, physiochemical stability, and biocompatibility. In addition, the diamond Solution-Gate Field-Effect Transistors (SGFETs) on the hydrogen-terminated diamond surface, which includes a conductive layer of p-type without doping, is suitable due to the lack of passivation layers and membranes on the channel surface. Here, we investigate simple and direct immobilization methods of DNAs for detection of single-mismatch oligonucleotide. The specific hybridization with 100nM concentrations of 21 base pair DNA can be clearly detected by two methods, fluorescence microscopy and diamond SGFETs. A single-mismatch oligonucleotide was successfully distinguished on diamond by controlling various factors, such as intrinsic hybridization affinity of target oligonucleotides including a single mismatch and negative surface charge of oxidized diamond surface in real-time detection.
 

Analytical Approaches
9:00 AM-12:00 PM, Thursday, August 23, 2007 BCEC -- 104A, Oral

Division of Analytical Chemistry

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007