Metabolic activities of novel inducible fermentations for recombinant therapeutic proteins

BIOT 80

Mark Berge, mberge@amgen.com and Radu Georgescu, radug@amgen.com. Cell Science and Technology, Amgen, One Amgen Center Drive, 18S-1-A, Thousand Oaks, CA 91320
Gaining an understanding of a fermentation process can take many forms, one of which is the study of metabolic process parameters. This study investigates the metabolic heat released with regards to oxygen uptake characteristics during the varied stages of fermentation processes used for the production of recombinant therapeutic proteins. This data set builds on previous metabolic heat determinations for batch processes, expanding the metabolic heat evolution data into fed and induction stages of the fermentation which have not been well represented in literature. The relationship of carbon consumption with regards to the oxygen consumption and heat evolution are also detailed.