BIOT 153 |
| A significant problem in mammalian cell culture is the direct assessment of the concentration and composition of the biomass. It is well recognized that considerable single-cell variability exists in a cell culture and that this heterogeneity affects culture performance. Therefore, an automated flow cytometry system was developed to further understand how culture heterogeneity changes. Automated flow cytometry can provide online cell number and single-cell data for which a flow cytometry stain exists. We show that this device can be used to detect the onset of apoptosis which can predict the onset of stationary phase by approximately 20 hours. Therefore, by passaging the culture at the onset of apoptosis, cell death is minimized and subsequently, culture scale-up is accomplished in an automated and efficient manner. Furthermore, online cell cycle staining can be accomplished, which yields very detailed information of the cell growth dynamics that can be used for subsequent control strategies. |
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Upstream Processing: Advances in Cell Culture Process Development
3:00 PM-5:25 PM, Tuesday, August 21, 2007 BCEC -- 107A, Oral
Division of Biochemical Technology |