Anticancer drug test in tissue engineered perfusion microbioreactor array

BIOT 456

Yuan Wen, wen.33@osu.edu1, Xudong Zhang, zhangx@chbmeng.ohio-state.edu2, and Shang-Tian Yang, yang.15@osu.edu2. (1) Chemical and Biomolecular Engineering, The Ohio State University, 125A Koffolt Labs, 140 West 19th Avenue, Columbus, OH 43210, (2) Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 West 19th Ave., Columbus, OH 43210
In vitro tissue culture provides a physiologically relevant platform for disease models and drug discovery. We have developed a 4-times-6 microfluidic bioreactor array which enabled perfusion culture of tissue constructs with the capability of real-time quantification of cell proliferation using EGFP (enhanced green fluorescent protein)-expressing cells. The effect of a commonly used chemotherapeutical drug 5-fluorouracil was investigated against a human colon cancer cell line HT-29. The drug was tested with clinically relevant modes, either in continuous perfusion or high dose bolus administration followed by perfusion culture. With this system, 6 concentrations of the drug could be investigated with 4 replicates plus controls simultaneously for hundreds of hours. Besides the real-time cell proliferation assay, direct access to the tissue constructs after the drug test also allowed further analysis of the anti-cancer mechanisms of the drug.
 

Upstream Processing: Advances in Tissue Engineering
8:00 AM-11:00 AM, Thursday, August 23, 2007 BCEC -- 107C, Oral

Division of Biochemical Technology

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007