Effects of scaffold porosity and pore size on cell entrapment, attachment, distribution and proliferation in 3-D cell culture system

BIOT 97

Robin Ng, ng.128@osu.edu, Department of Chemical Engineering, Ohio State University, 140 West 19th Ave., Koffolt Lab, Columbus, OH 43210 and Shang-Tian Yang, yang.15@osu.edu, Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 West 19th Ave., Columbus, OH 43210.
Current progress in three-dimensional culture produced a new culture system for biologic manufacturing in which scaffold's pore size and porosity are important parameters. The scaffold used in this study was nonwoven fibrous PET. Scaffolds with different porosities, ranging from 93% to 97%, and pore sizes, ranging from 27 µm to 70 µm, were prepared through hydrolysis in 1M NaOH at 65˚C. Our model predicted the effect of treatment time on pore size. We assessed the cell entrapment and distribution kinetics in the scaffolds using GFP-transfected ES cells and CHO-K1 cells. Cells distributed differently in scaffolds with different pore sizes: cells adsorbed on the fiber surface at a lower pore size and were entrapped in the void space between fibers at a higher. This difference in cell distribution led to the difference in cell attachment rate and cell proliferation. The pore sizes did not affect the growth rate of the cells.
 

Stem Cells: Engineering the Embryonic and Adult Stem Cell Niche
3:00 PM-5:00 PM, Monday, August 20, 2007 BCEC -- 108, Oral

Division of Biochemical Technology

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007