BIOL 20 |
| Robust and uniform oriented biomolecules immobilization techniques that preserve the activity of biomolecules have many potential applications such as microarray, micro-and nanoparticles, biosensor and medical device and thus are great challenge in biological research. In this presentation, we demonstrate that both aqueous Diels-Alder and click chemistry provide facile route for the immobilization of biomolecules such as protein and carbohydrate onto a solid surface in a chemically and biologically orthogonal manner and site-specific fashion. First, versatile cyclopentadiene-containing poly(ethylene glycol) (PEG) linkers was investigated for biotin, carbohydrate and protein immobilization onto a N-maleimide (EMC)-surface. Second, azide-containing carbohydrate and recombinant protein was immobilized onto an alkyne-terminated PEGylated surface. Successful immobilization of carbohydrates and recombinant protein was confirmed by confocal microscopy after FITC-labeled specific carbohydrate-protein and protein-antibody binding experiments. Our results indicated that aqueous Diels-Alder and Click chemistry reactions proceed to completion at low temperature and in aqueous solvents. In particular, cyclodiene, EMC, azide and alkyne, are stable under bioconjugation conditions and are not predisposed to unintended side reactions. |
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Frontiers in Chemical Biology
5:00 PM-7:00 PM, Sunday, August 19, 2007 BCEC -- Exhibit Hall - B2, Poster
Division of Biological Chemistry |