I&EC 71 |
| In this work, the effects of four additives (NaCl, sucrose, amylum, and dextrin) on the stability of β-mannanase (an important industry enzyme) during spray drying were discussed and the operational conditions were also optimized by response surface methodology (RSM). The experimental results of the above additives with various concentrations were shown that the addition of sucrose and dextrin might greatly enhance the thermal stability of enzyme during spray drying, and the maximum activity of β-mannanase was increased about 50 % when dextrin was 50 mg/ml. Furthermore, RSM was applied to optimization of three significant spray-drying parameters: feed flow rate, heated air flow rate and inlet temperature when dextrin was constant (50 mg/ml). Through RSM, the optimal conditions of spray drying were calculated as follows: feed flow rate of 4.3 mL/min, heated air flow rate of 31.3 L/h, and inlet temperature of 177 oC. Under these conditions, β-mannanase activity of 3734.9 U/g was experimentally obtained, which increased about 21.9 % comparing with that before optimization. |
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IEC Poster Session
8:00 PM-10:00 PM, Tuesday, August 21, 2007 BCEC -- Exhibit Hall - B2, Poster
Division of Industrial & Engineering Chemistry |