BIOT 438 |
| Recent advances in cell culture technology for monoclonal antibody production has resulted product (antibody) titers greater than 5 g/L. The increased titer coupled with a crude feed stream poses a challenge to the downstream steps consisting only of chromatography unit operations. We explored alternative purification processes using precipitation. Here we present results comparing the two purification schemes. The comparison attributes will include parameters such as product quality (aggregates/lower-molecular weight impurities by size-exclusion, host cell contaminant, DNA, etc.), scale-up as well as overall productivity. |
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Downstream Processing: Non-Chromatographic Separation Techniques: Improving Process Throughput
8:00 AM-11:00 AM, Thursday, August 23, 2007 BCEC -- 107B, Oral
Division of Biochemical Technology |