Engineering of an L-arabinose metabolic pathway in Corynebacterium glutamicum

BIOT 266

Miho Sasaki, mmg-lab@rite.or.jp, Hideo Kawaguchi, Shohei Okino, Toru Jyojima, Masayuki Inui, inui@rite.or.jp, and Hideaki Yukawa, mmg-lab@rite.or.jp. Microbiology Research Group, Research Institute of Innovative Technology for the Earth (RITE), 9-2 Kizugawadai, Kizu-shi, Kyoto, 619-0292, Japan
Lignocellulosic biomass from agricultural wastes represents abundant and cost-effective renewable energy source that are to date underutilized for production of ethanol and most chemicals. Lignocellulose is mainly composed of cellulose, and contains a significant amount of pentose-derived compounds, D-xylose and L-arabinose. To expand the catabolic properties of industrial corynebacteria with the objective to make possible the conversion of pentoses to a variety of compounds, we previously developed genetically modified strains of Corynebacterium glutamicum capable of utilizing D-xylose. In this study, C. glutamicum was metabolically engineered to broaden its substrate utilization range to include another pentose, L-arabinose. The resulting transformant exerted simultaneous utilization of glucose and L-arabinose.

This study was partially supported by a grant from New Energy and Industrial Technology Development Organization (NEDO).

 

Poster Session
5:30 PM-7:30 PM, Wednesday, August 22, 2007 BCEC -- Exhibit Hall - B2, Poster

Division of Biochemical Technology

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007