Isolation and identification of acetic acid bacteria from Shanxi superior mature vinegar in China

BIOT 264

Ning Yang, yanglvning80@163.com and Lin Hao, haolinsxnd@yahoo.com.cn. Department of Bioengineering, College of Food Science and Engineering, Shanxi Agricultural University, Shanxi, Taigu P.R. China, Taigu, Shanxi, AL 030801, China
Shanxi Superior Mature Vinegar is ranked the first position among Chinese four famous vinegar as its "special flavor and good quality" and its good color, savory and taste. Shanxi Superior Mature Vinegar collected a large amount of acetic acid bacteria over the course of many years' production process. The experimental sample came from the Shanxi Mature Vinegar Factory's solid-fermenter. By using the changing color circle method to separate Acetobacter spp., then by conducting Gram dyeing and defining experiments which produce acetic acid, the study finally isolated 176 strains of Acetobacter spp.. On the basis of their shape, cultivated traits, physiological and biochemical properties, and the determination of G+C mol% of DNA (GC ratio), the isolated strains were determined to belong to the following five species of Acetobacter, respectively: A. aceti (G+C mol%=64.1%), A. pasteurianus (G+C mol%=61.2%), A. hansenii (G+C mol%=59.2%), A. rancens (G+C mol%=57.5%) and A. liquefaciens (G+C mol%=55.4%).
 

Poster Session
5:30 PM-7:30 PM, Wednesday, August 22, 2007 BCEC -- Exhibit Hall - B2, Poster

Division of Biochemical Technology

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007