Reduction in high mannose glycoforms in mammalian cell culture through media optimization

BIOT 279

Nicole Le, nle@amgen.com, Cell Science and Technology, Amgen Inc, One Amgen Center Drive, Mailstop 18s-1-a, thousand oaks, CA 91320 and Bob Pendleton, Cell Science & Technology, Amgen, Inc, One Amgen Center Drive, thousand oaks, CA 91320.
The culture environment is a highly critical factor to maximize cell growth and product titer. Over the last years product quality and the ability to modulate it was of high interest to Amgen. We could demonstrate that various culture methods, process setpoints and some nutrient concentrations can have a profound effect on the oligosaccharide structures of glycoproteins. This presentation will discuss how the percentage of high mannose species of an antibody can be significantly reduced, even modulated utilizing medium optimization strategies without compromising the overall product titer.
 

Poster Session
5:30 PM-7:30 PM, Wednesday, August 22, 2007 BCEC -- Exhibit Hall - B2, Poster

Division of Biochemical Technology

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007