CARB 27 |
| Establishing a general and effective method for regulating gene expression in mammalian systems is important for many aspects of biological and biomedical research. Herein we report the antisense activities of cell-permeable, guanidine-based peptide nucleic acid (PNA) called GPNA. We show that GPNA designed to bind to the transcriptional start-site of human E-cadherin gene, induces potent and sequence-specific antisense effects, and is less toxic to the cells than the corresponding PNA-polyarginine conjugate. GPNA confers its silencing effect by blocking protein translation. The findings reported in this study provide a molecular framework for designing the next generation cell-permeable nucleic acid mimics for regulating gene expression in live cells and intact organisms. |
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Nucleic Acids as Drug Targets
1:40 PM-4:50 PM, Monday, August 20, 2007 BCEC -- 208, Oral
Division of Carbohydrate Chemistry |