ANYL 346 |
| High field asymmetric waveform ion mobility spectrometry (FAIMS) is a gas phase separation technique that has received widespread attention over the past few years. A major advantage of FAIMS is that separations are achieved in the millisecond timescale, which would be invaluable for improving sample throughput in a discovery pharmaceutical setting. This presentation explores the potential of FAIMS in conjunction with nanoelectrospray ionization for the rapid quantification of pharmacokinetic samples. FAIMS was used in this work to resolve matrix and metabolite interferences so they do not interfere with the determination of the analyte. Additional robustness was imparted to the method with the use of online nanoelectrospray ionization. Not only is nanoelectrospray known to be more tolerant to ion suppression, but the application of an online format allowed sample introduction and data acquisition to be completely automated. This experimental setup was tested with pharmacokinetic samples derived from Millennium's discovery programs. |
|
Analytical Technology for Drug Discovery
8:30 AM-11:50 AM, Tuesday, August 21, 2007 BCEC -- 105, Oral
Division of Analytical Chemistry |