Studies on activity and stability of recombinant tissue-type plasminogen activator

POLY 173

Jie Gang, gangjie1585@sina.com, College of Life Science, Dalian Nationalities University, Liaohe West Road 18#, Dalian, 116600, China, Chang Cao, College of life Sciences and Technology, Qiqihar University, Wenhua Road 30#, Qiqihar, 161006, China, and Jinhua Shao, College of Life Science and Technology, Qiqihar University, Wenhua Road 30#, Qiqihar, 161006, China.
In this paper, the activity and stability of recombinant tissue-type plasminogen activator (t-PA) from Trichoderma reesei effected by pH, metal ions and chemical reagents were discussed. The results indicate that the metal ions of Fe3+, Ca2+ and K+ promote the enzyme activity, but Zn2+ and Co2+ inhibit the enzyme activity. Furthermore the stability of the recombinant t-PA was improved by 30% sucrose.
 

General Papers: Functional Materials
6:00 PM-8:00 PM, Sunday, August 19, 2007 BCEC -- Exhibit Hall - B2, Poster

Division of Polymer Chemistry

The 234th ACS National Meeting, Boston, MA, August 19-23, 2007