BIOT 400 |
| 4,4-Difluoro-4-bora-3a,4a-diaza-s-indacene (boron-dipyrromethene, BDP or BODIPY) based fluorescence dyes have attracted a lot of attention in most recent years, for their high fluorescence quantum yields (&Phi f), relatively high molar absorption coefficients (&epsilon), good photostability, insensitivity towards solvent and pH, narrower spectrum in absorption and emission, and amenable to structural modification et al. This kind of dyes is suitable for probing the anions, cations and small molecules, and was applied to the biosensor, nucleic acid detection, pathological analysis and cell imaging also widely in present work. This research was undertaken to obtain a series of new BODIPY dyes' derivatives by substituted aldehydes or carboxylic acid anhydrides and pyrroles, which were further treated with N-hydroxysuccinimide. As we all known, fluorescence detection is widely used in analysis field for its high sensitivity and efficiency. One-pot reaction was applied and obtained the carboxylic BODIPY dyes in relatively high yield with different conditions. What's more, the activated BODIPY-NHS esters can be further used in biochemical analysis by its fluorescence spectrum properties. It should be noted that, the BODIPY dyes are well proper probes for investigating dye-DNA interactions, More research work has been taken on application in protein-labeling, such as BSA(bovine serum albumin) and so on.
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Poster Session
5:30 PM-7:30 PM, Wednesday, August 22, 2007 BCEC -- Exhibit Hall - B2, Poster
Division of Biochemical Technology |
