CHED 1099 |
| Grateloupia doryphora is an invasive rhodophyte that thrives in the intertidal region of many parts of the world. Extraction of carotenoids from G. doryphora was investigated to determine the method that would yield the greatest percentage. A methanol superficial extraction, bioassay of the extractant, and analysis using GC/MS were completed to ensure no interference with carotenoid extraction. This organic extraction showed that the algal surface was infused with petroleum products that inhibited bacterial growth. The carotenoid extraction was accomplished by blending the seaweed with MTBE and concentrating the sample by rotoevaporation. The sample was reconstituted in ether and passed through a short silica column. Elution with solvents of in increasing polarity from ether to methanol yielded fractions that were quantitatively analyzed by HPLC using a C30 column. The components were further separated by preparative TLC and characterized by FTIR, 1H and 13C 2D NMR, and HPLC/MS. |
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Undergraduate Research Poster Session: Environmental Chemistry
2:00 PM-4:00 PM, Monday, March 26, 2007 Hyatt Regency Chicago -- Riverside Center, Poster
Division of Chemical Education |